Human nasal epithelial cells cultured on a microchip react to air pollutants
Date: 27.10.2014
The upper respiratory tract is the first line of defense against air pollutants, including allergens, bacteria and environmental toxicants. Finger-like protrusions called cilia on the surface of the human mucous membrane, or epithelium, sway back and forth when irritated. This coordinated 'beating' movement of the cilia helps to remove foreign materials and is an important protective mechanism.
Wei Wang and Zhi Ping Wang at the A*STAR Singapore Institute of Manufacturing Technology, De Yun Wang at the National University of Singapore and co-workers have now developed the first microfluidic device that enables the direct observation of cilia and their beating frequency on a polyester membrane. The artificial system is used to observe the effects of air pollutants on cells in the upper airway.
The researchers constructed their microfluidic device using glass and a transparent, moldable polymer to ensure a clear view of the cilia and their activity. A membrane—designed to support the cultivation and differentiation of human nasal epithelial stem cells—was inserted into a small chamber on the device and fresh or contaminated air was fed through a tiny channel.
Five weeks after seeding the membrane with human nasal epithelial stem cells the researchers could observe the formation of beating cilia. The cilia beating frequency varied across samples but the difference was typically within a few hertz.
When fresh air was passed into the chamber, the researchers observed a 3 per cent drop in cilia beating frequency relative to the baseline value. In contrast, when air mixed with 0.5 milligrams per cubic meter of formaldehyde was passed through the chamber, they observed a 7.4 per cent increase in cilia beating frequency relative to the baseline value...
