In the fields of molecular biology and biochemistry, a reverse transcriptase, also known as RNA-dependent DNA polymerase, is a DNA polymerase enzyme that transcribes single-stranded RNA into single-stranded DNA.
It also is a DNA-dependent DNA polymerase which synthesizes a second strand of DNA complementary to the reverse-transcribed single-stranded cDNA after degrading the original mRNA. Normal transcription involves the synthesis of RNA from DNA; hence, reverse transcription is the reverse of this.
Human telomerase is also a reverse transcriptase.
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